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All customers need to register before they can use our services.

The LGTC is an internal facility of the Center for Human and Clinical Genetics and the Leiden University Medical Center (LUMC). After registration through the LGTC Order System, Human Genetic and LUMC-researchers can place orders to use our services.






SAVE THESE DATES!

3Gb test NGS Course: next-generation sequencing in a diagnostic setting
24 - 27 Oct. 2016
Lisbon, Portugal
Click here for more info



Variant Effect Prediction Training Course
31st Oct. - 3rd Nov. 2016
Heraklion, Crete, Greece
Click here for more info



14th International Symposium on Variants in the Genome: detection, sequencing & interpretation
5 - 8 June 2017
Santiago de Compostela, Spain

Key Info.
Earlybird Registration Ends 20th February 2017
Abstract Submission Closes 31st January 2017
Click here for more info




Sanger sequencing
Please deliver your Sanger Sequencing samples as usual in R4-21.
Recommended Concentration and Volume:
Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul).
Template_concentration is size related:
BAC concentration over 500ng/ul
Plasmid (over 4kb) concentration 200ng/ul
Plasmid (below 4kb) concentration 100-150ng/ul
Purified PCR product (over 700bp): higher than 50ng/ul
Purified PCR product (300bp ~ 700bp): 25-50ng/ul
Purified PCR product less than 300bp): 10-15ng/ul






» See publications of work we were involved in




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