LGTC Order System v.1.2.5 Updated 2019/05/17 
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Dear LGTC user, due to the measures taken against the Corona virus the LGTC will be closed until further notice. The Sanger sequencing service by Macrogen continues. If you have any questions please contact us by sending an email to LGTC@lumc.nl. The LGTC team

We have a new procedure for picking up Sanger sequencing tubes. Tubes and more information are available in room R5-20.

Sanger sequencing
Please deliver your Sanger Sequencing samples as usual in R4-21.
Tubes and caps are available at R5-20.

Recommended Concentration and Volume:

Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul).

Template_concentration is size related:
BAC concentration over 500ng/ul
Plasmid (over 4kb) concentration 200ng/ul
Plasmid (below 4kb) concentration 100-150ng/ul

Purified PCR product (>1.5kb): 100ng/ul
Purified PCR product (700bp ~ 1.5kb): 50-75ng/ul
Purified PCR product (300bp ~ 700bp): 25-50ng/ul
Purified PCR product less than 300bp): 10-15ng/ul

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