LGTC Order System v.1.2.5 Updated 2019/05/17 
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We have a new procedure for picking up Sanger sequencing tubes. Tubes and more information are available in room R5-20.

Sanger sequencing
Please deliver your Sanger Sequencing samples as usual in R4-21.
Tubes and caps are available at R5-20.

Recommended Concentration and Volume:

Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul).

Template_concentration is size related:
BAC concentration over 500ng/ul
Plasmid (over 4kb) concentration 200ng/ul
Plasmid (below 4kb) concentration 100-150ng/ul

Purified PCR product (>1.5kb): 100ng/ul
Purified PCR product (700bp ~ 1.5kb): 50-75ng/ul
Purified PCR product (300bp ~ 700bp): 25-50ng/ul
Purified PCR product less than 300bp): 10-15ng/ul

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