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Ion Torrent PGM ("Personal Genome Machine")
The LGTC has added one Ion Torrent PGM system to its repertoire of next generation sequencers. In the future we hope to make this machine available for departments within the LUMC and Leiden University to use it on their own after a mandatory introduction (training) from the LGTC.
Technology
The PGM is a desktop sequence machine that uses disruptive semiconductors sensors to give a real-time measurement of hydrogen ions that are released during DNA template extension. A high-density array of wells on the Ion semiconductor chips provides millions of individual reactors while an integrated fluidics system allows reagents to flow over the sensor array. The direct measurement of hydrogen ions circumvents the need for fluorescent labeled nucleotides and time consuming imaging, thus reducing sequencing time.
For now the PGM is able to sequence ~100 bp DNA fragments (or amplicons) in less than 3 hours with an output of 10 Mb per 314 chip. This will quickly improve over time to longer fragments up to 400 bp and a 1 Gb output.
More information about the Ion Torrent PGM can be found here.
Applications
- Small genome sequencing
- amplicon sequencing
- CAGE/SAGE sequencing
- RNAseq
Using the Ion Torrent PGM
Under construction.
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