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Illumina BeadXpress
System
The Leiden Genome Technology Center has an Illumina BeadXpress system. It is a dual-color laser detection system that enables scanning of multiplexed assays (up to 384 per sample) using the VeraCode glass bead technology.
Assays
- Genotyping
- GoldenGate kits
- Universal Capture Bead sets
- Carboxyl Bead sets
- Gene expression
- Methylation profiling
- Protein analysis
See the Illumina website for more information on the Illumina BeadXpress and the VeraCode technology, assays and types of beads, custom and standard.
Illumina BeadXpress at the LGTC
Projects
When you have a project for the Illumina BeadXpress and have not used the system before it is necessary to discuss it with the LGTC. We can help you discuss and evaluate the possibilities, costs, planning, and all other issues.
Quotation
Once it has been decided how to start you will need a quotation from the LGTC. This quotation can be requested via the LGTC order system for which you will need a valid account. A quotation for Illumina BeadXpress experiments contains the following products: Illumina order charge, BeadXpress beads, consumables and reagents needed for the BeadXpress GoldenGate assay, and scanning of the samples done by yourself. To accept the costs you need to deliver a printed and signed version of the quotation to the LGTC. Only then, the project will start. You will need to be trained on the system by a LGTC technician and will need to make a separate appointment for the laboratory training. Without a quotation we will not train you on the system.
Preparation of samples
All protocols are available on the Illumina website, you will need to register to access these protocols or ask the LGTC to download them for you.
Planning and time span
The planning is up to you.
Data and data analysis
The quotation does not include data analysis since the LGTC will not do the data analysis for you. Copy your data to an external hard drive. In all the cases: we will not keep your data for longer than three months, it is your responsibility to make your own back ups.
The LGTC has a data-analysis PC available for Illumina BeadXpress users with Illuminas latest version of GenomeStudio installed. You need to be authorized to get access to this computer, there is also a Yahoo calendar available to make reservations.
Summary: what can you expect from us? General rules
- It is your responsibility to follow the protocols described by Illumina. When in doubt ask the LGTC. The LGTC is not responsible for any failed runs due to any reasons. Beads will not be refunded and not re-run for free.
- When the hybridizations are done by the LGTC you will receive the raw data on a DVD. We will only keep your data for three months; it is your own responsibility to make back up copies of the data.
- You are responsible for the data management of your experiments. With the raw data you can do all data analysis you want. The latest version of GenomeStudio is available on a LGTC computer and free to use. Do not store any data on the LGTC computers.
- All discounts, special wishes, planning, complaints, problems, etc... have to be discussed with and agreed by the lab-manager or they will not be considered valid.
More information? Questions?
Go to www.illumina.com.
Contact the LGTC.
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