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Correcting for multiple testing in Rosetta
Ellen Sterrenburg <E.sterrenburg@lumc.nl>
2003-09-25 17:59:08
Hi Wayne,

I was wondering if the p-value that I get out of ANOVA in Rosetta is
corrected for multiple testing?
Do you know that or do we have to do that afterwards?
Thanks!

Best Regards,

Ellen
Hi Ellen

Thank you for the explanation. Here is what our head statistician had to
say about the correction for multiple tests:

Currently, Resolver does not provide multiple test correction, such as
Bonferroni correction. If a user is interested in this, it can be computed
easily:

1) For a given alfa and N multiple tests (# of sequences in the analysis),
the new p-value threshold with Bonferroni correction is alfa/N.
2) For the example above, alfa=0.01 and N=10,000, if we choose
p-value<0.01/10,000=0.000001, we will have random chance less than 0.01 of
any one sequence being mistakenly detected.

Bonferroni correction is extremely conservative. It kills the detection
sensitivity. People should use it with caution.



I hope that answers your question. Let me know if it does not.

Best regards,
Wayne
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Title
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Date
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