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Heat 4053:Select genes with signal in at least one exp. for your ANOVA
Ellen Sterrenburg <>
2003-04-30 07:47:48
Hi Wayne,

I did my data analysis on a time-course experiment.
I performed ANOVA with 5 groups and 2 hybs per group.
I got genes that were significant (p < 0.01).
When I was checking some of these genes it appeared that there was a gene
that was not (or hardly visible) expressed during the whole time-course. In
other words, the ratios don't say much in this case.
Is it possible to define somewhere that for your ANOVA you want only genes
that have a certain tresh-hold value for the intensity for at least one
timepoint? And that tresh-hold value should then be a value that is some
standard deviations above background.
Thanks in advance!

Best regards,


Hi Ellen

There is no "one-button" method of doing this but you can certainly select
sequences from a table and then broadcast this selection to the ANOVA plot.

To do this, bring up a table of the sequences in one of the experiments of
your ANOVA. Click on the 'Intensity 1' column heading to sort by this
field. You can then 'click' and 'shift-click' to select everything in the
range of intensities that you are interested in. Once they are selected,
click 'Ctrl-B' to broadcast this to an open ANOVA window.

After this, you can choose 'Groups|Selection|Hide Others' in the ANOVA plot
to show only sequences that are above the threshold. This should allow you
to view only the signature sequences with intensities above a threshold.

Can I also ask a favor of you? I am starting to make some changes to the
way I do support for Resolver users in Europe with the eventual goal of
improving the level of support. One of the first steps of this is having
all support issues sent to . This probably won't
have many immediately noticable effects but it will be the first step and
will allow others to see your inquiries if I am unavailable.

Thank you,
[reply to this message]

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