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Dye-swaps and combining hybs
Ellen Sterrenburg <E.sterrenburg@lumc.nl>
2003-04-04 16:09:04
Hi Wayne,

I have loaded 20 profiles into Rosetta.
These are pairs of 2 (dye-swaps).
For the analysis, I want to assign the dye-swap to these profiles.
On-the-fly analysis gives this option, but when I want to do ANOVA, this is
not possible.
Do I have to do this before I load the profiles, because something like that
is possible in the hybmanager or is it also possible to do it afterwards?

And when I combined two hybs in one experiment, the two original profiles
were lost and replaced by one experiment.
Is it also possible to add extra the combination of the two and keep the
originals?

Ellen


Hi Ellen

You are right that there is nowhere to change the polarity of a ratio
experiment when submitting it to the ANOVA. You will need to do this first
in the Ratio Hyb Manager as you suggested. The thing to note, though, is
that changing the polarity in the Hyb Manager will only affect the
'experiment'; the 'profile' will remain with the same polarity as that is
the raw data. You might think of the experiment as being the data from the
profile plus the information specified in the Hyb. Does that make any
sense? :)

By the same idea, when you combine two profiles using 'Hybs to Combine', the
two profiles should still be there but, indeed, there will now be only one
experiment. This is related to an often requested feature that a single
profile can be present in more than one experiment (in it's own experiment
and in the combined experiment in your case). This feature will be part of
Resolver v4.0, which is expected to be available in a couple of months. I
hope you can work around this limitation until then.

Best regards,
Wayne
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[new message]
Title
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Date
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2003-04-03 17:56:18
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2003-04-03 17:54:08
Dye-swaps and combining hybs
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2003-04-04 16:09:04
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