|LGTC Order System v.1.1.6||Updated 2013/09/25|
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2017-06-01: Sanger sequences delayed
Macrogen has indicated they are experiencing technical difficulties processing the Sanger Samples. They are working on solving the issue and are going to deliver the results as soon as possible.
Apologies for the delay.
ZonMw and NWO have made funds available for the 4th call for projects within the ZonMw programme Enabling Technologies Hotels.
Click here too visit the LGTC TECHNOLOGY HOTEL
Please deliver your Sanger Sequencing samples as usual in R4-21.
Tubes and caps are available at R5-20.
Recommended Concentration and Volume:
Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul).
Template_concentration is size related:
BAC concentration over 500ng/ul
Plasmid (over 4kb) concentration 200ng/ul
Plasmid (below 4kb) concentration 100-150ng/ul
Purified PCR product (over 700bp): higher than 50ng/ul
Purified PCR product (300bp ~ 700bp): 25-50ng/ul
Purified PCR product less than 300bp): 10-15ng/ul
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