LGTC LIMS System v.1.1.6 Updated 2013/09/25 
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2017-02-17: Sanger sequences delayed
Over 1000 samples have been submitted in the last few of days.
Macrogen is working hard to get the data to you asap.

Apologies for the delay.

Sanger sequencing
Please deliver your Sanger Sequencing samples as usual in R4-21.
Tubes and caps are available at R5-20.

Recommended Concentration and Volume:
Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul).
Template_concentration is size related:
BAC concentration over 500ng/ul
Plasmid (over 4kb) concentration 200ng/ul
Plasmid (below 4kb) concentration 100-150ng/ul
Purified PCR product (over 700bp): higher than 50ng/ul
Purified PCR product (300bp ~ 700bp): 25-50ng/ul
Purified PCR product less than 300bp): 10-15ng/ul

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