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Next-Gen Sequencing
Micro-array technology
Sanger sequencing & Fragment analysis
Liquid handling / Robotics
Real-Time PCR and Melting Curve Analysis
General equipment
Miscellaneous equipment
To use many of the LGTC equipment, you'll need to book your time on a Yahoo calendar.
The passwords are here
.
Next-Gen Sequencing
Genome Analyzer IIx incl. Paired-End module (2x)
Illumina Next-Gen Sequencing for DNA sequencing, transcriptome sequencing, miRNA profiling, ChIP-SEQ, sequence capture.
HiSeq 2000 Illumina
The Hiseq 2000 is the new next generation sequencer from Illumina to follow up the GAII. It can now hold two flowcell in one machine. Each flowcell can be scanned on two sides, increasing the amount of output per lane. With a 2x 100 bp reads the Hiseq can produce up to 200 Gb in ~8 days. The flowcells can operate independently from each other with different protocols and with separate reagents.
Ion Torrent PGM ("Personal Genome Machine")
The PGM is a desktop sequence machine that uses disruptive semiconductors sensors to give a real-time measurement of hydrogen ions that are released during DNA template extension. A high-density array of wells on the Ion semiconductor chips provides millions of individual reactors while an integrated fluidics system allows reagents to flow over the sensor array. The direct measurement of hydrogen ions circumvents the need for fluorescent labeled nucleotides and time consuming imaging, thus reducing sequencing time.
For now the PGM is able to sequence ~100 bp DNA fragments (or amplicons) in less than 3 hours with an output of 10 Mb per 314 chip. This will quickly improve over time to longer fragments up to 400 bp and a 1 Gb output.
Helicos Genetic Analysis System
Helicos Genetic Analysis System, including a Heliscope Single Molecule Sequencer (HSMS), a HeliScope analysis engine and two Heliscope sample loaders.
Roche 454
Genome sequencer FLX 454 Life sciences.
Micro-array technology
FlexArrayer
Design and synthesize custom micro-arrays. On-glass synthesis of oligonucleotides using lasers. The arrays are suitable for a wide range of genetic analyses.
BeadArray 500GX
For Illumina Gene Expression and Genotyping BeadChips.
Beadarray station and scanner, including side loader.
Agilent scanner
G2565BA microarray scanner for scanning of any brand 1 x 3 inch microarrays in Cy3 and Cy5 channel with 5 or 10 µm resolution.
Book your time
(in-house only) and bring a signed order report (
LGTC order system
)
First time user training:
To scan your first slides, please contact us in advance so we can assist you. This training is obligatory.
Sanger sequencing & Fragment analysis
ABI PRISM® 3730xl Analyzer
96 capillary sequencer. For Sanger sequencing reaction and any kind of fragment analysis.
Liquid handling / Robotics
Liquidator 96
96 multi channel manual pipetting system for 96- and 384-well plates.
Sciclone ALH 3000 Liquid Handling Workstation
Automated liquid handling workstation for any automated liquid handling application, from 1 - 200µl in 96 or 384 well format. Includes a spectrophotometer and a PCR machine.
Tecan EVO150 (dedicated for Illumina BeadChips)
Robotic workstation dedicated for the processing of Illumina BeadChips.
Real-Time PCR and Melting Curve Analysis
Fluidigm Biomark System
Real-time nanoliter PCR system for Gene Expression and Genotyping (96x96 or 48x48 dynamic array) and target quantitation (digital array). Melting Curve Analysis will soon be available on this system.
Book your time
(in-house only)
Lightcycler LC-480
Real-Time PCR system for Gene Expression and Genotyping, 96- and 384- well plates based. High Resolution Melting Curve Analysis available.
7900HT Real Time PCR System
Real-Time PCR system for Gene Expression and Genotyping, 96- and 384- well plates based. Also equiped for Taqman Low Density Arrays. High throughput with a plate handling robot.
Book your time
(in-house only)
Lightscanner
High Resolution Melting for mutation scanning and genotyping in a 96- and 384-well plate system.
General equipment
Agilent 2100 Bioanalyzer
Lab-on-a-Chip system for automated electrophoresis of DNA, RNA and protein samples. Note: We no longer provide protein chips.
First time user training:
To run your first chip, please contact us in advance so we can assist you. This training is obligatory.
Book your time
(in-house only) and bring a signed order report (
LGTC order system
)
Caliper LabChip 90
High throughput lab-on-a-chip system for electrophoresis of DNA, RNA and protein in 96- and 384- well format. Note: We do not provide protein chips on a regular basis.
First time user training:
To run your first chip, please contact us in advance so we can assist you. This training is obligatory.
Book your time
(in-house only) and bring a signed order report (
LGTC order system
)
NanoDrop ND-1000
UV-Vis Spectrophotometer to quantify µl samples of DNA, RNA and protein.
Covaris S-series Single Tube
For a broad range of applications:
DNA shearing, Chromatin shearing, Tissue disruption and homogenization, Sample extraction for metabolic and proteomic profiling, RNA extraction, Cell lysis, Compound dissolution, Compound formulation, ADME/Tox.
Caliper LabChip XT
The Caliper XT can perform automated electrophoresis with the option to size select (range 10bp to 500bp, peak width 5-20% ) and collect your DNA. The DNA is collect in a sequence ready buffer, so no need for purification. Especially next generation sequencing will benefit from this machine as it speeds up sample preparation by removing any of the gel steps.
First time user training:
To run your first chip, please contact us in advance so we can assist you. This training is obligatory.
Book your time
(in-house only) and bring a signed order report (
LGTC order system
)
Miscellaneous equipment
Pyrosequencing AB - PSQ™ 96 System
Pyrosequencing system.
Applied Biosystems - ABI PRISM® 3730 Analyzer
48 capillary sequencer.
Genomic Solutions - Omnigrid 100 micro-Arrayer
Microarray spotter.
Beckman Coulter - BIOMEK 2000 robotic workstation, SideLoader system
Available for performing large numbers of clone and plate transfers (incl. high-density gridding), DNA-preparations and pipetting runs.
Genomic Solutions - GeneTAC G3 Robotic Workstation
For colony picking, library copying, re-arraying (making clone sub-collections).
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